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Nutrient Agar Kit
(Dehydrated)
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HIGHEST QUALITY LABORATORY GRADE INGREDIENTS AVAILABLE
Nutrient Agar is a microbiological growth medium commonly used for the routine cultivation of non-fastidious bacteria. It is useful because it remains solid even at relatively high temperatures. Also, bacteria grown in nutrient agar grows on the surface, and is clearly visible as small colonies.
Kit Includes:
*16 grams Dehydrated Nutrient Agar
*20, 100mm x 15mm Sterilized Plastic (polystyrene) Petri Dishes with Lids
*20 Sterile Cotton Swabs
Each Kit will yield approximately 360ml of Nutrient Agar Solution which will yield 20-25, 100mm Petri Dishes.
FORMULA:
21.7% Pancreatic Digest of Gelatin (Peptone G)
13% Beef Extract
65.2% Agar Agar
SOLUTION INSTRUCTIONS:
Step 1: Dissolve Nutrient Agar in 600ml of Distilled Water
Step 2: Bring to a Boil & Stir
Step 3: Filter Sterilize or Autoclave at 121 degrees Celsius for 15 minutes
Step 4: Pour into Petri Dishes
Step 5: Enjoy & Record your Experiments!
FREQUENTLY ASKED QUESTIONS (FAQ):
Q/ What is an Autoclave & What does it do?
A/ An autoclave is a device that heats the nutrient agar under pressure in order to sterilize it prior to inoculation. An autoclave is essentially a pressure cooker. You can substitute a household pressure cooker for an autoclave. Many modern household pressure cookers will not reach 250 degrees Fahrenheit at 15PSI so you may have to increase the amount of time you "cook" the nutrient agar to insure sterility is achieved.
Q/ Do I have to sterilize the Nutrient Agar?
A/ Sterilization is highly recommended but not required. Many would-be scientists have successful experiments without the use of an autoclave and without sterilizing the nutrient agar, after all, the objective is generally to grow mold and / or bacteria which will occur either way.
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